qmt_sirfse: qMT using Inversion Recovery Fast Spin Echo acquisition¶

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Contents

% This m-file has been automatically generated using qMRgenBatch(qmt_sirfse)
% for publishing documentation.
% Command Line Interface (CLI) is well-suited for automatization
% purposes and Octave.
%
% Please execute this m-file section by section to get familiar with batch
% processing for qmt_sirfse on CLI.
%
% Demo files are downloaded into qmt_sirfse_data folder.
%
% Written by: Agah Karakuzu, 2017
% ==============================================================================

1. Print qmt_sirfse information

qMRinfo('qmt_sirfse');
  qmt_sirfse:  qMT using Inversion Recovery Fast Spin Echo acquisition
Pulse Sequence Diagram

ASSUMPTIONS:
(1) FILL
(2)
(3)
(4)

Inputs:
MTdata              Magnetization Transfert data
(R1map)             1/T1map (OPTIONAL but recommended)
(Mask)              Binary mask to accelerate the fitting (OPTIONAL)

Outputs:
F                   Ratio of number of restricted pool to free pool, defined
as F = M0r/M0f = kf/kr.
kr                  Exchange rate from the free to the restricted pool
(note that kf and kr are related to one another via the
definition of F. Changing the value of kf will change kr
accordingly, and vice versa).
R1f                 Longitudinal relaxation rate of the free pool
(R1f = 1/T1f).
R1r                 Longitudinal relaxation rate of the restricted pool
(R1r = 1/T1r).
Sf                  Instantaneous fraction of magnetization after vs. before
the pulse in the free pool. Starting point is computed using Block
simulation.
Sr                  Instantaneous fraction of magnetization after vs. before
the pulse in the restricted pool. Starting point is computed using block
simulation.
M0f                 Equilibrium value of the free pool longitudinal
magnetization.
(M0r)               Equilibrium value of the restricted pool longitudinal
magnetization. Computed using M0f = M0r * F.
(kf)                Exchange rate from the restricted to the free pool.
Computed using kf = kr * F.
(resnorm)           Fitting residual.

Protocol:
MTdata
Ti                Inversion times (s)
Td                Delay times (s)

FSEsequence
Trf               Duration of the pulses in the FSE sequence (s)
Tr                Delay between the pulses in the FSE sequnece (s)
Npulse            Number of refocusing pulses in the FSE sequence

Options:
Inversion Pulse
Shape             Shape of the inversion pulse.
Available shapes are:
- hard
- gaussian
- gausshann (gaussian pulse with Hanning window)
- sinc
- sinchann (sinc pulse with Hanning window)
- singauss (sinc pulse with gaussian window)
- fermi
Duration          Duration of the inversion pulse (s)

Fitting
Use R1map to      By checking this box, you tell the fitting
constrain R1f       algorithm to check for an observed R1map and use
its value to constrain R1f. Checking this box
will automatically set the R1f fix box to true in
the Fit parameters table.
Fix R1r = R1f     By checking this box, you tell the fitting
algorithm to fix R1r equal to R1f. Checking this
box will automatically set the R1r fix box to
true in the Fit parameters table.

Sr Calculation
Lineshape         The absorption lineshape of the restricted pool. Available lineshapes are: Gaussian, Lorentzian and SuperLorentzian.
T2r               Transverse relaxation time of the restricted pool (T2r = 1/R2r)

Example of command line usage:
For more examples: qMRusage(qmt_sirfse)

Author: Ian Gagnon, 2017

Reference page in Doc Center
doc qmt_sirfse


2. Setting model parameters

2.a. Create qmt_sirfse object

Model = qmt_sirfse;

2.b. Set protocol and options

Protocol: MRI acquisition parameters that are accounted for by the respective model.

For example: TE, TR, FA FieldStrength. The assigned protocol values are subjected to a sanity check to ensure that they are in agreement with the data attributes.

Options: Fitting preferences that are left at user's discretion.

For example: linear fit, exponential fit, drop first echo.

2.b.1 Set protocol the CLI way

If you are using Octave, or would like to serialize your operations any without GUI involvement, you can assign protocol directly in CLI:

Ti = [0.0030; 0.0037; 0.0047; 0.0058; 0.0072; 0.0090; 0.0112; 0.0139; 0.0173; 0.0216; 0.0269; 0.0335; 0.0417; 0.0519; 0.0646; 0.0805; 0.1002; 0.1248; 0.1554; 0.1935; 0.2409; 0.3000; 1.0000; 2.0000; 10.0000];
% Ti is a vector of [25X1]
Td = [3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000; 3.5000];
% Td is a vector of [25X1]
Model.Prot.MTdata.Mat = [ Ti Td];
Trf  = 0.001;
Tr  = 0.01;
Npulse = 16;
Model.Prot.FSEsequence.Mat = [ Trf  Tr  Npulse];

  See the generic notes section below for further information.

2.b.2 Set protocol and options the GUI way

The following command opens a panel to set protocol and options (if GUI is available to the user):

Model = Custom_OptionsGUI(Model);

If available, you need to close this panel for the remaining of the script to proceed.

  Using this panel, you can save qMRLab protocol files that can be used in both interfaces. See the generic notes section below for details.

3. Fit MRI data

3.a. Load input data

This section shows how you can load data into a(n) qmt_sirfse object.

  • At the CLI level, qMRLab accepts structs containing (double) data in the fields named in accordance with a qMRLab model.

  See the generic notes section below for BIDS compatible wrappers and scalable
      qMRLab workflows.

%          |- qmt_sirfse object needs 3 data input(s) to be assigned:
%          |-   MTdata
%          |-   R1map
%          |-   Mask

data = struct();
% MTdata.nii.gz contains [128  128    1   25] data.
data.MTdata=double(load_nii_data('qmt_sirfse_data/MTdata.nii.gz'));
% Mask.nii.gz contains [128  128] data.
data.Mask=double(load_nii_data('qmt_sirfse_data/Mask.nii.gz'));

3.b. Execute fitting process

This section will fit the loaded data.

FitResults = FitData(data,Model,0);

Visit the generic notes section below for instructions to accelerate fitting by
      parallelization using ParFitData.

3.c. Display FitResults

You can display the current outputs by:

qMRshowOutput(FitResults,data,Model);

A representative fit curve will be plotted if available.

To render images in this page, we will load the fit results that had been saved before. You can skip the following code block;

% Load FitResults that comes with the example dataset.
FitResults_old = load('FitResults/FitResults.mat');
qMRshowOutput(FitResults_old,data,Model);

3.d. Save fit results

Outputs can be saved as *.nii.(gz) if NIfTI inputs are available:

% Generic function call to save nifti outputs
FitResultsSave_nii(FitResults, 'reference/nifti/file.nii.(gz)');

If not, FitResults.mat file can be saved. This file contains all the outputs as workspace variables:

% Generic function call to save FitResults.mat
FitResultsSave_mat(FitResults);

  FitResults.mat files can be loaded to qMRLab GUI for visualization and ROI
       analyses
.

The section below will be dynamically generated in accordance with the example data format (mat or nii). You can substitute FitResults_old with FitResults if you executed the fitting using example dataset for this model in section 3.b..

FitResultsSave_nii(FitResults_old, 'qmt_sirfse_data/MTdata.nii.gz');

3.e. Re-use or share fit configuration files

qMRLab's fit configuration files (qmt_sirfse_Demo.qmrlab.mat) store all the options and protocol in relation to the used model and the release version.

  *.qmrlab.mat files can be easily shared with collaborators to allow them fit their own
      data or run simulations using identical option and protocol configurations.

Model.saveObj('my_qmt_sirfse_config.qmrlab.mat');

4. Simulations

4.a. Single Voxel Curve

Simulates single voxel curves
  1. Analytically generate synthetic MRI data
  2. Add rician noise
  3. Fit and plot the respective curve

      x = struct;
x.F = 0.1;
x.kr = 30;
x.R1f = 1;
x.R1r = 1;
x.Sf = -0.98419;
x.Sr = 0.65638;
x.M0f = 1;
% Set simulation options
Opt.SNR = 50;
Opt.Method = 'Analytical equation';
Opt.T2fUsedinBlockequation = 0.04;
% run simulation
figure('Name','Single Voxel Curve Simulation');
FitResult = Model.Sim_Single_Voxel_Curve(x,Opt);

4.b. Sensitivity Analysis

Simulates sensitivity to fitted parameters
  1. Iterate fitting parameters from lower (lb) to upper (ub) bound
  2. Run Sim_Single_Voxel_Curve for Nofruns times
  3. Compute the mean and std across runs

            F             kr            R1f           R1r           Sf            Sr            M0f
      OptTable.st = [0.1           30            1             1             -0.98         0.66          1]; % nominal values
OptTable.fx = [0             1             1             1             1             1             1]; %vary F...
OptTable.lb = [0.0001        0.0001        0.05          0.05          -1            0.0001        0.0001]; %...from 0.0001
OptTable.ub = [1             1e+02         10            10            0             1             2]; %...to 1
% Set simulation options
Opt.SNR = 50;
Opt.Method = 'Analytical equation';
Opt.T2fUsedinBlockequation = 0.04;
Opt.Nofrun = 5;
% run simulation
SimResults = Model.Sim_Sensitivity_Analysis(OptTable,Opt);
figure('Name','Sensitivity Analysis');
SimVaryPlot(SimResults, 'F' ,'F' );

5. Notes

5.a. Notes specific to qmt_sirfse

Not provided.

5.b. Generic notes

5.b.1. Batch friendly option and protocol conventions

If you would like to load a desired set of options / protocols programatically, you can use *.qmrlab.mat files. To save a configuration from the protocol panel of qmt_sirfse, first open the respective panel by running the following command in your MATLAB command window (MATLAB only):

Custom_OptionsGUI(qmt_sirfse);

In this panel, you can arrange available options and protocols according to your needs, then click the save button to save my_qmt_sirfse.qmrlab.mat file. This file can be later loaded into a qmt_sirfse object in batch by:

Model = qmt_sirfse;
Model = Model.loadObj('my_qmt_sirfse.qmrlab.mat');

  Model.loadObj('my_qmt_sirfse.qmrlab.mat') call won't update the fields in the Model object, unless the output is assigned to the object as shown above. This compromise on convenience is to retain Octave CLI compatibility.

If you don't have MATLAB, hence cannot access the GUI, two alternatives are available to populate options:

  1. Use qmrlab/mcrgui:latest Docker image to access GUI. The instructions are available here.
  2. Set options and protocols in CLI:
  • List available option fields using tab completion in Octave's command prompt (or window)
Model = qmt_sirfse;
Model.option. % click the tab button on your keyboard and list the available fields.
  • Assign the desired field. For example, for a mono_t2 object:
Model = mono_t2;
Model.options.DropFirstEcho = true;
Model.options.OffsetTerm = false;

Some option fields may be mutually exclusive or interdependent. Such cases are handled by the GUI options panel; however, not exposed to the CLI. Therefore, manual CLI options assignments may be challenging for some involved methods such as qmt_spgr or qsm_sb. If above options are not working for you and you cannot infer how to set options solely in batch, please feel free to open an issue in qMRLab and request the protocol file you need.

Similarly, in CLI, you can inspect and assign the protocols:

Model = qmt_sirfse;
Model.Prot. % click the tab button on your keyboard and list the available fields.

Each protocol field has two subfields of Format and Mat. The first one is a cell indicating the name of the protocol parameter (such as EchoTime (ms)) and the latter one contains the respective values (such as 30 x 1 double array containing EchoTimes).

The default Mat protocol values are set according to the example datasets served via OSF.

5.b.2 Parallelization

Beginning from release 2.5.0, you can accelerate fitting for the voxelwise models using parallelization.

Available in MATLAB only. Requires parallel processing toolbox.

In CLI, you can perform parallel fitting by:

parpool();
FitResults = ParFitData(data,Model);

If a parpool exists, the ParFitData will use it. If not, a new pool will be created using the local profile. By default, ParFitData saves outputs automatically every 5 minutes. You can disable this feature by:

FitResults = ParFitData(data, Model, 'AutosaveEnabled', false);

Alternatively, you can change the autosave interval (min 1 min) by:

FitResults = ParFitData(data,Model,'AutoSaveInterval',10);

If something went wrong during the fitting (e.g. your computer had to be restarted), you can recover the autosaved data by:

FitResults = ParFitData(data,Model,'RecoverDirectory','/ParFitTempResults_*/folder/from/the/previous/session');

GUI users will be prompted a question about whether they would like to use parallelization after clicking the Fit Data button, if the conditions are met. When called from GUI, ParFitData will be run with default options:

  • Save temporary results every 5 minutes or whenever a chunk has finished processing
  • Split data into chunks with a granularity factor of 3
  • Do not remove temporary fit results upon completion

For further information:

help ParFitData

The default parallelization options can be changed in the preferences.json file located at the root qMRLab directory.

6. Citations

qMRLab JOSS article

Karakuzu A., Boudreau M., Duval T.,Boshkovski T., Leppert I.R., Cabana J.F., Gagnon I., Beliveau P., Pike G.B., Cohen-Adad J., Stikov N. (2020), qMRLab: Quantitative MRI analysis, under one umbrella 10.21105/joss.02343

Reference article for qmt_sirfse

Li, K., et al. (2010). Optimized inversion recovery sequences for quantitative T1 and magnetization transfer imaging. Magnetic Resonance in Medicine, 64(2), 491-500. 10.1002/mrm.22440


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